Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using NCK1 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using NCK1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE diluted at 1:1000| Product Name | NCK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NCK1 (NCK adaptor protein 1)The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NCK1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, Sheep, Rhesus Monkey, Chimpanzee, BovineMouse (98%), Rat (99%), Xenopus laevis (84%), Pig (97%), Sheep (98%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC12668 antibody NCK antibody NCKalpha antibody nck-1 antibody NCK1 antibody cytoplasmic protein NCK1 antibody NCK tyrosine kinase antibody SH2/SH3 adaptor protein NCK-alpha antibody melanoma NCK protein antibody non-catalytic region of tyrosine |
|---|---|
| Molecular Weight(MW) | 43 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Endoplasmic reticulum |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using NCK1 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using NCK1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE diluted at 1:1000| Positive Control | K562 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
