Application
NCK1 Antibody (N-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of NCK1 Antibody (N-term) in Hela cell line lysates (35ug/lane). NCK1 (arrow) was detected using the purified Pab.| Product Name | NCK1 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NCK1 (N-term)The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 9~38 amino acids from the N-terminal region of human NCK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SH2/SH3 adaptor protein NCK-alpha SH2/SH3 adaptor protein NCKalpha NCK adaptor protein1 Nck1 NCK1 NCK Cytoplasmic protein NCK1 NCK adaptor protein 1 Nck1 NCK1 NCK adaptor protein 1 Nck-1 NCK adaptor protein 1 Nck 1 |
|---|---|
| Target | NCK1 |
| Molecular Weight(MW) | 42.864 kDa (note) |
| Cellular Localization | Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments. |
Application
NCK1 Antibody (N-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of NCK1 Antibody (N-term) in Hela cell line lysates (35ug/lane). NCK1 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
