Application
Western blot analysis of NCK1 Antibody (N-term) in Hela cell line lysates (35ug/lane). NCK1 (arrow) was detected using the purified Pab.Application
NCK1 Antibody (N-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NCK1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NCK1; NCK; Cytoplasmic protein NCK1; NCK adaptor protein 1; SH2/SH3 adaptor protein NCK-alpha |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NCK |
|---|---|
| Alternative Names | NCK1 NCK Cytoplasmic protein NCK1 NCK adaptor protein 1 SH2/SH3 adaptor protein NCK-alpha |
| Molecular Weight(MW) | 42864 Da |
| Function | Adapter protein which associates with tyrosine- phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling |
| Tissue Specificity | This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~38 amino acids from the N-terminal region of human NCK1. |
| Cellular Localization | Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments |
| Entrez Gene | 4690 |
|---|
Application
Western blot analysis of NCK1 Antibody (N-term) in Hela cell line lysates (35ug/lane). NCK1 (arrow) was detected using the purified Pab.
Application
NCK1 Antibody (N-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
