Application
NCK1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NCK1 antibody detected the NCK1 protein (arrow).Application
Western blot analysis of NCK1 (arrow) using rabbit polyclonal NCK1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCK1 gene.Application
NCK1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NCK1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NCK1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NCK1; NCK; Cytoplasmic protein NCK1; NCK adaptor protein 1; SH2/SH3 adaptor protein NCK-alpha |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NCK |
|---|---|
| Alternative Names | NCK1 NCK Cytoplasmic protein NCK1 NCK adaptor protein 1 SH2/SH3 adaptor protein NCK-alpha |
| Molecular Weight(MW) | 42864 Da |
| Function | Adapter protein which associates with tyrosine- phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling |
| Tissue Specificity | This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-92 amino acids from the N-terminal region of human NCK1. |
| Cellular Localization | Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments |
| Entrez Gene | 4690 |
|---|---|
| Nucleotide Accession | NP_006144.1 NP_001177725.1 |
Application
NCK1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NCK1 antibody detected the NCK1 protein (arrow).
Application
Western blot analysis of NCK1 (arrow) using rabbit polyclonal NCK1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCK1 gene.
Application
NCK1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NCK1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
