Application
A. Western blot analysis on Hela cell lysate using anti-Nck RabMAb (Cat. #AJ1527a) 1:10,000 dilution.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Nck RabMAb (Cat. #AJ1527a).| Product Name | Nck Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NCK1, NCK, Cytoplasmic protein NCK1, SH2/SH3 adaptor protein NCK-alpha |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y531 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NCK |
|---|---|
| Alternative Names | NCK1 NCK Cytoplasmic protein NCK1 SH2/SH3 adaptor protein NCK-alpha |
| Molecular Weight(MW) | 42864 Da |
| Function | Adapter protein which associates with tyrosine- phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling |
| Tissue Specificity | A synthetic peptide corresponding to residues close to the SH2 domain of human Nck was used as immunogen. |
| Cellular Localization | Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments |
| Entrez Gene | 4690 |
|---|
Application
A. Western blot analysis on Hela cell lysate using anti-Nck RabMAb (Cat. #AJ1527a) 1:10,000 dilution.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Nck RabMAb (Cat. #AJ1527a).| Application Notes | WB:1:500~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
