Application
NCKAP1 antibody [C1C2], Internal detects NCKAP1 protein by Western blot analysis. A. 50 µg rat brain lysate/extract 5 % SDS-PAGE NCKAP1 antibody [C1C2], Internal dilution: 1:1000Application
Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:1000Application
Sample (30μg whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500| Product Name | NCKAP1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NCKAP1 (NCK-associated protein 1)Is part of lamellipodial complex that controls Rac-dependent actin remodeling. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NCKAP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Rhesus MonkeyZebrafish (97%), Xenopus laevis (96%), Rhesus Monkey (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ11291 antibody HEM2 antibody KIAA0587 antibody MGC8981 antibody NAP1 antibody NAP125 antibody NCKAP1 antibody membrane-associated protein HEM-2 antibody nck-associated protein 1 antibody p125Nap1 antibody NAP 1 antibody |
|---|---|
| Molecular Weight(MW) | 129 kDa (note) (2) |
| Cellular Localization | Cell membrane; Single-pass membrane protein; Cytoplasmic side (By similarity) , Cell projection , lamellipodium membrane; Single-pass membrane protein; Cytoplasmic side (By similarity) |
Application
NCKAP1 antibody [C1C2], Internal detects NCKAP1 protein by Western blot analysis. A. 50 µg rat brain lysate/extract 5 % SDS-PAGE NCKAP1 antibody [C1C2], Internal dilution: 1:1000
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE diluted at 1:1000
Application
Sample (30μg whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500| Positive Control | HepG2 , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
