Application
NCS1 antibody detects NCS1 protein at on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NCS1 antibody dilution: 1:500.Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using NCS1 antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.Application
Sample(30 μg of whole cell lysate) A:A431 B:Hep G2 12% SDS PAGE diluted at 1:200| Product Name | NCS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NCS1 (neuronal calcium sensor 1)This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NCS1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, BovineRat (100%), Xenopus laevis (99%), Chicken (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp761L1223 antibody FLUP antibody FREQ antibody NCS1 antibody frequenin-like protein antibody neuronal calcium sensor 1 antibody frequenin-like ubiquitous protein antibody frequenin homolog antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) |
| Cellular Localization | Golgi apparatus , Golgi stack membrane; Peripheral membrane protein |
Application
NCS1 antibody detects NCS1 protein at on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NCS1 antibody dilution: 1:500.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using NCS1 antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.
Application
Sample(30 μg of whole cell lysate) A:A431 B:Hep G2 12% SDS PAGE diluted at 1:200| Positive Control | A431 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
