Application
SLC8A1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
SLC8A1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC8A1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of SLC8A1 Antibody (Center) in HL-60(lane 1), K562(lane 2) cell line lysates (35ug/lane). SLC8A1 (arrow) was detected using the purified Pab.| Product Name | NCX1 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NCX1 (Internal)In cardiac myocytes, Ca(2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca(2+) concentration during contraction is primarily due to release of Ca(2+) from intracellular stores. However, some Ca(2+) also enters the cell through the sarcolemma (plasma membrane). During relaxation, Ca(2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca(2+) that entered across the sarcolemma must be extruded from the cell. The Na(+)-Ca(2+) exchanger is the primary mechanism by which the Ca(2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation.[supplied by OMIM] |
| Immunogen | KLH conjugated synthetic peptide between 303~333 amino acids from the Center region of human SLC8A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Na(+)/Ca(2+)-exchange protein 1 Sodium/calcium exchanger 1 Na(+)/Ca(2+)exchange protein1 Sodium/calcium exchanger1 SLC8A1 SLC8A1 CNC NCX1 Na(+)/Ca(2+)exchange protein 1 |
|---|---|
| Target | SLC8A1 |
| Molecular Weight(MW) | 108.547 kDa (note) |
Application
SLC8A1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
SLC8A1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC8A1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of SLC8A1 Antibody (Center) in HL-60(lane 1), K562(lane 2) cell line lysates (35ug/lane). SLC8A1 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
