Application
Sample (50 ug of whole cell lysate) A: mouse liver 15% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HepG2, using NDUFA12 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA12 antibody at 1:500 dilution.Application
NDUFA12 antibody [N1C3] detects NDUFA12 protein at mitochondria in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. NDUFA12 antibody [N1C3] diluted at 1:500.| Product Name | NDUFA12 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NDUFA12 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12)Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Immunogen | Full length human NDUFA12 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Sheep, Rhesus Monkey, Chimpanzee, BovineSheep (89%), Rhesus Monkey (94%), Chimpanzee (100%), Bovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | B17.2 antibody DAP13 antibody NDUFA12 antibody complex I B17.2 subunit antibody CI-B17.2 antibody complex I-B17.2 antibody CIB17.2 antibody 13 kDa differentiation-associated protein antibody NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subuni |
|---|---|
| Molecular Weight(MW) | 17 kDa (note) |
| Cellular Localization | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side |
Application
Sample (50 ug of whole cell lysate) A: mouse liver 15% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HepG2, using NDUFA12 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA12 antibody at 1:500 dilution.
Application
NDUFA12 antibody [N1C3] detects NDUFA12 protein at mitochondria in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. NDUFA12 antibody [N1C3] diluted at 1:500.| Positive Control | HepG2 , Raji , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
