Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA5 antibody at 1:500 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE diluted at 1:1000Application
NDUFA5 antibody [N1C3] detects NDUFA5 protein at mitochondria in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. NDUFA5 antibody [N1C3] diluted at 1:500.| Product Name | NDUFA5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NDUFA5 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa)The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. [provided by RefSeq] |
| Immunogen | Full length human NDUFA5 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, Chimpanzee, BovineRat (80%), Rhesus Monkey (94%), Chimpanzee (100%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | B13 antibody CI-13KD-B antibody CI-13kB antibody DKFZp781K1356 antibody FLJ12147 antibody NUFM antibody UQOR13 antibody NDUFA5 antibody type I dehydrogenase antibody NADH-ubiquinone oxidoreductase 13 kDa-B subunit antibody Complex I-13KD-B antibo |
|---|---|
| Molecular Weight(MW) | 13 kDa (note) (2) |
| Cellular Localization | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side |
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA5 antibody at 1:500 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE diluted at 1:1000
Application
NDUFA5 antibody [N1C3] detects NDUFA5 protein at mitochondria in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. NDUFA5 antibody [N1C3] diluted at 1:500.| Positive Control | H1299 , HeLa , HepG2 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
