Application
NDUFAB1 antibody detects NDUFAB1 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: NDUFAB1 protein stained by NDUFAB1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NDUFAB1 antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000Application
NDUFAB1 antibody detects NDUFAB1 protein by Western blot analysis. A. 30 ug C8D30 whole cell lysate/extract 12 % SDS-PAGE NDUFAB1 antibody dilution: 1:1000Application
NDUFAB1 antibody detects NDUFAB1 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12 % SDS-PAGE NDUFAB1 antibody dilution: 1:5000| Product Name | NDUFAB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NDUFAB1 (NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1, 8kDa)Carrier of the growing fatty acid chain in fatty acid biosynthesis in mitochondria. Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NDUFAB1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rhesus Monkey, Chimpanzee, BovinePig (81%), Rhesus Monkey (92%), Chimpanzee (99%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACP antibody FASN2A antibody MGC65095 antibody SDAP antibody NDUFAB1 antibody mitochondrial acyl carrier protein antibody complex I SDAP subunit antibody CI-SDAP antibody NADH:ubiquinone oxidoreductase SDAP subunit antibody NADH-ubiquinone oxidore |
|---|---|
| Molecular Weight(MW) | 17 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
NDUFAB1 antibody detects NDUFAB1 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: NDUFAB1 protein stained by NDUFAB1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NDUFAB1 antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000
Application
NDUFAB1 antibody detects NDUFAB1 protein by Western blot analysis. A. 30 ug C8D30 whole cell lysate/extract 12 % SDS-PAGE NDUFAB1 antibody dilution: 1:1000
Application
NDUFAB1 antibody detects NDUFAB1 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12 % SDS-PAGE NDUFAB1 antibody dilution: 1:5000| Positive Control | 293T , C8D30 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
