Application
Immunofluorescence analysis of methanol-fixed HeLa, using NDUFB5 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using NDUFB5 antibody at 1:500 dilution.Application
NDUFB5 antibody detects NDUFB5 protein by Western blot analysis. A.30 µg 293T whole cell lysate/extract 12 % SDS-PAGE NDUFB5 antibody dilution: 1:1000Application
NDUFB5 antibody detects NDUFB5 protein by Western blot analysis. A. 50 µg mouse brain lysate/extract 12 % SDS-PAGE NDUFB5 antibody dilution: 1:1000Application
NDUFB5 antibody detects NDUFB5 protein at cytoplasm in rat brian by immunohistochemical analysis. Sample: Paraffin-embedded rat brian. NDUFB5 antibody diluted at 1:500.Application
NDUFB6 antibody detects NDUFB6 protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. NDUFB6 antibody diluted at 1:500.| Product Name | NDUFB5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NDUFB5 (NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa)The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NDUFB5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, Chimpanzee, BovineRhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CISGDH antibody DKFZp686N02262 antibody FLJ30597 antibody MGC111204 antibody MGC12314 antibody SGDH antibody NDUFB5 antibody NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5 mitochondrial antibody complex I-SGDH antibody NADH-ubiquinon |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) (2) |
| Cellular Localization | Mitochondrion inner membrane; Single-pass membrane protein; Matrix side |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using NDUFB5 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using NDUFB5 antibody at 1:500 dilution.
Application
NDUFB5 antibody detects NDUFB5 protein by Western blot analysis. A.30 µg 293T whole cell lysate/extract 12 % SDS-PAGE NDUFB5 antibody dilution: 1:1000
Application
NDUFB5 antibody detects NDUFB5 protein by Western blot analysis. A. 50 µg mouse brain lysate/extract 12 % SDS-PAGE NDUFB5 antibody dilution: 1:1000
Application
NDUFB5 antibody detects NDUFB5 protein at cytoplasm in rat brian by immunohistochemical analysis. Sample: Paraffin-embedded rat brian. NDUFB5 antibody diluted at 1:500.
Application
NDUFB6 antibody detects NDUFB6 protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. NDUFB6 antibody diluted at 1:500.| Positive Control | 293T , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
