Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:10000Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: JurKat D: Raji 10% SDS PAGE diluted at 1:1000Application
NDUFS2 antibody detects NDUFS2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: NDUFS2 protein stained by NDUFS2 antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NDUFS2 antibody at 1:250 dilution.Application
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NDUFS2 antibody diluted at 1:1000.| Product Name | NDUFS2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NDUFS2 (NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase))Mitochondrial complex I (NADH-ubiquinone reductase; EC 1.6.5.3) is the first multimeric complex of the respiratory chain that catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mammalian mitochondrial complex I is an assembly of at least 43 different subunits. Seven of the subunits are encoded by the mitochondrial genome; the remainder are the products of nuclear genes. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits, including NDUFS2. See NDUFS1 (MIM 157655).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NDUFS2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Rhesus Monkey, Chimpanzee, BovineZebrafish (84%), Xenopus laevis (90%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CI-49 antibody NDUFS2 antibody complex 1 mitochondrial respiratory chain 49-KD subunit antibody NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 mitochondrial antibody NADH-ubiquinone oxidoreductase 49 kDa subunit antibody complex I 49kDa subu |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (3) |
| Cellular Localization | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side |
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:10000
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: JurKat D: Raji 10% SDS PAGE diluted at 1:1000
Application
NDUFS2 antibody detects NDUFS2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: NDUFS2 protein stained by NDUFS2 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NDUFS2 antibody at 1:250 dilution.
Application
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with NDUFS2 antibody diluted at 1:1000.| Positive Control | 293T , A431 , Jurkat , Raji , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
