Application
NDUFS3 antibody detects NDUFS3 protein at mitochondria by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: NDUFS3 protein stained by NDUFS3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
NDUFS3 antibody detects NDUFS3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody diluted by 1:1000.Application
NDUFS3 antibody detects NDUFS3 protein at cytosol on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. NDUFS3 antibody dilution: 1:500.Application
NDUFS3 antibody detects NDUFS3 protein at cytosol on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. NDUFS3 antibody dilution: 1:500.| Product Name | NDUFS3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NDUFS3 (NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase))This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NDUFS3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineRat (90%), Zebrafish (80%), Xenopus laevis (83%), Pig (93%), Chicken (90%), Rhesus Monkey (97%), Chimpanzee (97%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CI-30 NADH dehydrogenase (ubiquinone) Fe-S protein 3 30kDa (NADH-coenzyme Q reductase) |
|---|---|
| Target | NDUFS3 |
| Molecular Weight(MW) | 30 kDa (note) |
| Cellular Localization | Mitochondrion inner membrane |
Application
NDUFS3 antibody detects NDUFS3 protein at mitochondria by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: NDUFS3 protein stained by NDUFS3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
NDUFS3 antibody detects NDUFS3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody diluted by 1:1000.
Application
NDUFS3 antibody detects NDUFS3 protein at cytosol on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. NDUFS3 antibody dilution: 1:500.
Application
NDUFS3 antibody detects NDUFS3 protein at cytosol on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. NDUFS3 antibody dilution: 1:500.| Positive Control | Jurkat , Raji , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
