Application
Human Breast (formalin-fixed, paraffin-embedded) stained with NEDD4 antibody at 1:100-1:200 followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Product Name | NEDD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NEDD4E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Is involved in ubiquitination of ERBB4 intracellular domain E4ICD. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. |
| Immunogen | GST fusion protein corresponding to the WW2 domain (aa395-462) of rat Nedd4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB, IHC-P (1:100 - 1:200): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Nedd4a nedd 4 protein nedd4 protein |
|---|---|
| Tissue Specificity | Recognizes rat Nedd4, Mr 115kD. Degradation products were also detected at Mr 55 and 60kD. Species cross-reactivity: mouse and human. |
Application
Human Breast (formalin-fixed, paraffin-embedded) stained with NEDD4 antibody at 1:100-1:200 followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Application Notes | WB, IHC-P (1:100 - 1:200): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Storage Buffer | 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide and 5mg/ml BSA before the addition of glycerol to 30% |
