Application
NEDD4L antibody detects NEDD4L protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NEDD4L antibody diluted at 1:1000.| Product Name | NEDD4L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NEDD4L (neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NEDD4L. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevisMouse (97%), Rat (97%), Xenopus laevis (89%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | neural precursor cell expressed developmentally down-regulated 4-like E3 ubiquitin protein ligase RSP5 hNEDD4-2 NEDD4.2 NEDD4-2 |
|---|---|
| Target | NEDD4L |
| Molecular Weight(MW) | 112 kDa (note) (10) |
| Cellular Localization | Cytoplasm |
Application
NEDD4L antibody detects NEDD4L protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NEDD4L antibody diluted at 1:1000.| Positive Control | PC-3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
