Application
WB analysis of human cystic fibrosis epithelial cells using NEDD4L antibody at 1:3000.Application
WB analysis of PANC1 cells using NEDD4L antibody at 0.2-1μg/ml.| Product Name | NEDD4L antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NEDD4LThis gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] |
| Immunogen | A synthetic peptide corresponding to an Internal region of Human NEDD4L |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HNEDD42 NEDD4.2 RSP5 NEDD4L NEDD42 |
|---|---|
| Target | NEDD4L |
| Molecular Weight(MW) | 112 KDa (note) |
Application
WB analysis of human cystic fibrosis epithelial cells using NEDD4L antibody at 1:3000.
Application
WB analysis of PANC1 cells using NEDD4L antibody at 0.2-1μg/ml.| Positive Control | cystic fibrosis epithelial cells , PANC1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
