Application
Nek1 antibody detects Nek1 protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nek1 protein stained by Nek1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Nek1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Nek1 (NIMA-related kinase 1)The protein encoded by this gene is a serine/threonine kinase involved in cell cycle regulation. The encoded protein is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Nek1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (97%), Rat (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SRPS2 NEK1 SRPS2A NIMA-related kinase 1 NY-REN-55 |
|---|---|
| Target | Nek1 |
| Molecular Weight(MW) | 143 kDa (note) |
| Cellular Localization | Nucleus |
Application
Nek1 antibody detects Nek1 protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nek1 protein stained by Nek1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
