Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NEK11 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:2000| Product Name | NEK11 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NEK11 (NIMA (never in mitosis gene a)- related kinase 11)This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NEK11. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ23495 antibody NEK11 antibody serine/threonine-protein kinase Nek11 antibody NIMA (never in mitosis gene a)- related kinase 11 antibody |
|---|---|
| Molecular Weight(MW) | 74 kDa (note) (7) |
| Cellular Localization | Nucleus , nucleolus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NEK11 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:2000| Positive Control | A549 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
