Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NEK3 antibody at 1:500 dilution.Application
Sample (30μg whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:1000| Product Name | NEK3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NEK3 (NIMA (never in mitosis gene a)-related kinase 3)This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NEK3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (88%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HSPK36 antibody MGC29949 antibody NEK3 antibody HSPK 36 antibody glycogen synthase A kinase antibody serine/threonine-protein kinase Nek3 antibody hydroxyalkyl-protein kinase antibody phosphorylase B kinase kinase antibody nimA-related protein kina |
|---|---|
| Molecular Weight(MW) | 58 kDa (note) (4) |
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NEK3 antibody at 1:500 dilution.
Application
Sample (30μg whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:1000| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
