Application
NEK4 antibody [N2C1], Internal detects NEK4 protein at nucleus by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 15 min. Green: NEK4 protein stained by NEK4 antibody [N2C1], Internal diluted at 1:500. Blue: Hoechst 33343 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using NEK4 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 7.5% SDS PAGE diluted at 1:1000| Product Name | NEK4 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NEK4 (NIMA (never in mitosis gene a)-related kinase 4) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NEK4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC33171 antibody NRK2 antibody STK2 antibody pp12301 antibody NEK4 antibody serine/threonine protein kinase-2 antibody serine/threonine-protein kinase Nek4 antibody serine/threonine-protein kinase 2 antibody nimA-related protein kinase 4 antibody |
|---|---|
| Molecular Weight(MW) | 95 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
NEK4 antibody [N2C1], Internal detects NEK4 protein at nucleus by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 15 min. Green: NEK4 protein stained by NEK4 antibody [N2C1], Internal diluted at 1:500. Blue: Hoechst 33343 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using NEK4 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 7.5% SDS PAGE diluted at 1:1000| Positive Control | A431 , H1299 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
