Application
Immunoprecipitation (IP) of NEK6 by using TrueMab monoclonal anti-NEK6 antibodies (Negative control: IP without adding anti-NEK6 antibody.). For each experiment, 500ul of DDK tagged NEK6 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEK6 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-NEK6 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NEK6mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-NEK6mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-NEK6mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-NEK6mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-NEK6mouse monoclonal antibody. ( Dilution 1:50)| Product Name | NEK6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2H7] to NEK6Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation. |
| Immunogen | Protein expressed in 293T cell transfected with human NEK6 expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2H7 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PIPWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SID6-1512 |
|---|---|
| Target | NEK6 |
| Tissue Specificity | is specific for human NEK6. |
Application
Immunoprecipitation (IP) of NEK6 by using TrueMab monoclonal anti-NEK6 antibodies (Negative control: IP without adding anti-NEK6 antibody.). For each experiment, 500ul of DDK tagged NEK6 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEK6 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-NEK6 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NEK6mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-NEK6mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-NEK6mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-NEK6mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-NEK6mouse monoclonal antibody. ( Dilution 1:50)| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
