Application
NEK7 antibody [C2C3], C-term detects NEK7 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NEK7 protein stained by NEK7 antibody [C2C3], C-term diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample(30 μg of whole cell lysate) A:HeLa S3 12% SDS PAGE diluted at 1:500Application
NEK7 antibody [C2C3], C-term detects NEK7 protein at cytoplasm on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NEK7 antibody [C2C3], C-term diluted at 1:500.| Product Name | NEK7 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NEK7 (NIMA (never in mitosis gene a)-related kinase 7)NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NEK7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus MonkeyRat (100%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NEK7 antibody nimA-related protein kinase 7 antibody serine/threonine-protein kinase Nek7 antibody never in mitosis A-related kinase 7 antibody NIMA (never in mitosis gene a)-related kinase 7 antibody |
|---|---|
| Molecular Weight(MW) | 35 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
NEK7 antibody [C2C3], C-term detects NEK7 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NEK7 protein stained by NEK7 antibody [C2C3], C-term diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample(30 μg of whole cell lysate) A:HeLa S3 12% SDS PAGE diluted at 1:500
Application
NEK7 antibody [C2C3], C-term detects NEK7 protein at cytoplasm on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NEK7 antibody [C2C3], C-term diluted at 1:500.| Positive Control | HeLaS3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
