Application
Western blot analysis of Nephrin in mouse kidney tissue lysate with Nephrin antibody at 1 ug/mL in the (A) absence and (B) presence of blocking peptide.Application
Immunohistochemistry of Nephrin in mouse kidney tissue with Nephrin antibody at 1 ug/mL.Application
Immunofluorescence of Nephrin in Mouse Kidney cells with Nephrin antibody at 10 ug/mL.| Product Name | Nephrin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NephrinThis gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009] |
| Immunogen | Nephrin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Nephrin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 1 ug/ml. IHC-P: 1 ug/ml. IF: 10 ug/ml: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | nephrosis 1 congenital Finnish type (nephrin) NPHN nephrin NPHS1 CNF |
|---|---|
| Target | Nephrin |
Application
Western blot analysis of Nephrin in mouse kidney tissue lysate with Nephrin antibody at 1 ug/mL in the (A) absence and (B) presence of blocking peptide.
Application
Immunohistochemistry of Nephrin in mouse kidney tissue with Nephrin antibody at 1 ug/mL.
Application
Immunofluorescence of Nephrin in Mouse Kidney cells with Nephrin antibody at 10 ug/mL.| Application Notes | WB: 1 ug/ml. IHC-P: 1 ug/ml. IF: 10 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
