Application
Nesprin 2 antibody [C3], C-term detects Nesprin 2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Nesprin 2 protein stained by Nesprin 2 antibody [C3], C-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 5% SDS PAGE diluted at 1:2000Application
Syne2 antibody [C3], C-term detects Syne2 protein at nucleus by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Syne2 protein stained by Syne2 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | Nesprin 2 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Nesprin 2 (synaptic nuclear envelope 2) |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of mouse Nesprin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | BovineBovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | nesprin 2 antibody synaptic nuclear envelope protein 2 antibody nuclear envelope spectrin repeat protein 2 antibody nesprin-2 antibody nuance antibody nucleus and actin connecting element protein antibody 6820443O06Rik antibody AW546258 antibody D1 |
|---|---|
| Molecular Weight(MW) | 783 kDa (note) |
Application
Nesprin 2 antibody [C3], C-term detects Nesprin 2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Nesprin 2 protein stained by Nesprin 2 antibody [C3], C-term diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 5% SDS PAGE diluted at 1:2000
Application
Syne2 antibody [C3], C-term detects Syne2 protein at nucleus by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Syne2 protein stained by Syne2 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
