Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000.Application
Various whole cell extracts (60 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nestin antibody diluted at 1:1000.| Product Name | Nestin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NestinMay play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse Nestin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AA166324 antibody C78523 antibody ESTM46 antibody Nes antibody |
|---|---|
| Target | Nestin |
| Molecular Weight(MW) | 207 kDa (note) |
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000.
Application
Various whole cell extracts (60 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nestin antibody diluted at 1:1000.| Positive Control | SK-N-AS , HUVEC , PC-3 , SK-N-SH , IMR32 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
