Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody [GT623] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody [GT623] diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Nestin antibody [GT623] detects Nestin protein at cytoskeleton by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nestin protein stained by Nestin antibody [GT623] diluted at 1:250. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | Nestin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT623] to Nestin (nestin)Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Nestin. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT623 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | nestin NES Nbla00170 |
|---|---|
| Target | Nestin |
| Molecular Weight(MW) | 177 kDa (note) |
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody [GT623] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody [GT623] diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Nestin antibody [GT623] detects Nestin protein at cytoskeleton by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nestin protein stained by Nestin antibody [GT623] diluted at 1:250. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | SK-N-SH , PC-3 , IMR32 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
