Application
Immunofluorescence analysis of methanol-fixed MCF-7, using Netrin G1 ligand antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Netrin G1 ligand antibody at 1:250 dilution.Application
Sample (30 µg of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Netrin G1 ligand antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Netrin G1 ligand (leucine rich repeat containing 4C)NGL1 is a specific binding partner for netrin G1 (NTNG1; MIM 608818), which is a member of the netrin family of axon guidance molecules (Lin et al., 2003 [PubMed 14595443]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Netrin G1 ligand. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, ZebrafishMouse (100%), Rat (100%), Zebrafish (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | KIAA1580 antibody NGL-1 antibody NGL1 antibody LRRC4C antibody leucine-rich repeat-containing protein 4C antibody netrin-G1 ligand antibody leucine rich repeat containing 4C antibody |
|---|---|
| Molecular Weight(MW) | 72 kDa (note) (3) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Immunofluorescence analysis of methanol-fixed MCF-7, using Netrin G1 ligand antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Netrin G1 ligand antibody at 1:250 dilution.
Application
Sample (30 µg of whole cell lysate) A: MCF-7 7.5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
