Application
NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 rat brain. Green: NeuN protein stained by NeuN antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
NeuN antibody detects NeuN protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: NeuN protein stained by NeuN antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .Application
NeuN antibody detects NeuN protein at nucleus by immunofluorescent analysis. Sample: Rat E18 primary cortical neuron, DIV 8 cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: NeuN protein stained by NeuN antibody diluted at 1:250. Green: alpha Tubulin, stained by alpha Tubulin antibody [GT114] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NeuN antibody diluted at 1:1000.| Product Name | NeuN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NeuN (RNA binding protein, fox-1 homolog (C. elegans) 3) |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human NeuN. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | MouseRat |
| Predicted Reactivity | Human, Rhesus MonkeyHuman (100%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FOX3 antibody HRNBP3 antibody NeuN antibody NeuN antibody RBFOX3 antibody RNA binding protein fox-1 homolog (C. elegans) 3 antibody |
|---|---|
| Target | NeuN |
| Molecular Weight(MW) | 34 kDa (note) |
Application
NeuN antibody detects NeuN protein at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 rat brain. Green: NeuN protein stained by NeuN antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
NeuN antibody detects NeuN protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: NeuN protein stained by NeuN antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .
Application
NeuN antibody detects NeuN protein at nucleus by immunofluorescent analysis. Sample: Rat E18 primary cortical neuron, DIV 8 cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: NeuN protein stained by NeuN antibody diluted at 1:250. Green: alpha Tubulin, stained by alpha Tubulin antibody [GT114] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NeuN antibody diluted at 1:1000.| Positive Control | mouse Retina , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
