Application
Neuron Specific Nuclear Protein (NeuN) antibody [2Q158] detects NeuN protein on rat cerebellumn by IHC-P analysis.(Citrate Buffer, pH 6.0.) Dilution:1:100 Immunoreactivity is seen as nuclear staining in the neurons in the granular layer.Application
Neuron Specific Nuclear Protein (NeuN) antibody [2Q158] detects NeuN protein on mouse brain(dentate gyrus and subventricular zone) by IHC-P analysis.(Citrate Buffer, pH 6.0.)| Product Name | NeuN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to NeuNVertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. NeuN (Neuronal Nuclei) is a neuron-specific, DNA-binding nuclear protein in vertebrates. In mice, NeuN is observed in most neuronal cell types throughout the nervous system, including cerebellum, cerebral cortex, hippocampus, thalamus, and spinal cord, as well as the dorsal root ganglia, sympathetic chain ganglia, and enteric ganglia of the peripheral nervous system. NeuN immunoreactivity is first observed in neurons when they become post-mitotic and are initiating cellular and morphological differentiation. No staining is observed in proliferative zones. NeuN has been used as an immunohistochemical marker for excitotoxic lesions of the brain, as well as in the diagnosis of a wide range of human tissue specimens from the central and peripheral nervous systems. |
| Immunogen | Purified cell nuclei from mouse brain |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| WB:1:500~1:1000 IHC:1:50~1:100 ICC/IF:1:100~1:500 |
|
| Species Reactivity | HumanMouseRatFerretSalamander |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NeuN RNA binding protein fox1 homolog (C. elegans) 3 HRNBP3 fox1 homolog C FLJ58356 hexaribonucleotide binding protein3 hexaribonucleotide binding protein 3 neuronal nuclei OTTHUMP00000196558 FOX-3 FLJ56884 fox-1 homolog C Neuron Specific Nucl |
|---|---|
| Target | NeuN |
| Tissue Specificity | Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei). Recognizes 2-3 bands in the 46-48kD range and possibly another band at approximately 66kD |
Application
Neuron Specific Nuclear Protein (NeuN) antibody [2Q158] detects NeuN protein on rat cerebellumn by IHC-P analysis.(Citrate Buffer, pH 6.0.) Dilution:1:100 Immunoreactivity is seen as nuclear staining in the neurons in the granular layer.
Application
Neuron Specific Nuclear Protein (NeuN) antibody [2Q158] detects NeuN protein on mouse brain(dentate gyrus and subventricular zone) by IHC-P analysis.(Citrate Buffer, pH 6.0.)| Application Notes | WB:1:500~1:1000 IHC:1:50~1:100 ICC/IF:1:100~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.4 containing 0.1% sodium azide and 40% glycerol |
