| Product Name | Neurabin 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Neurabin 2Spinophilin/Neurabin-II has been found to be enriched at the postsynaptic density fraction in the brain and in the adherens junction fraction in rat liver. Within the brain, Spinophilin/Neurabin-II is localized in the heads of dendritic spines. Spinophilin/Neurabin-II contains a single PDZ domain in the middle region of the protein and other domains that predict it will form a coiled-coil structure at the C-terminus. Spinophilin/Neurabin-II is known to complex with the catalytic subunit of protein phosphatase I (PP1) and in vitro is a potent modulator of PP1 enzymatic activity. Spinophilin/Neurabin-II is also known to interact with F-actin and rat lin-10. |
| Immunogen | GST fusion protein, corresponding to internal sequence amino acids 286-390 of Rat brain Neurabin 2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP: 4 μg immunoprecipitates Neurabin 2 from 500 μg of rat brain microsomal preparation. WB: 0.25 - 1 μg/ml detected Neurabin 2 in 20 μg of a rat brain microsomal preparation. Detects a band of approximately 120 kDa (predicted molecular weight: 89 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | Neurabin2 84686 Neurabin 2 Neb2 Ppp1r9b |
|---|---|
| Target | Neurabin 2 |
| Tissue Specificity | Specific for rat spinophilin at 120kD. Also recognizes a band at ~95 kDa which is suspected to be a cleavage product of neurabin 2. |
| Cellular Localization | Enriched at synapse and cadherin based cell to cell adhesion sites. |
| Positive Control | Rat brain microsomal preparation. |
|---|---|
| Application Notes | IP: 4 μg immunoprecipitates Neurabin 2 from 500 μg of rat brain microsomal preparation. WB: 0.25 - 1 μg/ml detected Neurabin 2 in 20 μg of a rat brain microsomal preparation. Detects a band of approximately 120 kDa (predicted molecular weight: 89 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide. Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4 |
