Application
Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
U87-MG whole cell extract and conditioned medium (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Neuregulin-1 antibody detects Neuregulin-1 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Neuregulin-1 protein stained by Neuregulin-1 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.Application
Sample (50 µg of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Neuregulin-1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Neuregulin-1Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NRG1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARIA antibody GGF antibody GGF2 antibody HGL antibody HRG antibody HRG1 antibody HRGA antibody MST131 antibody NDF antibody SMDF antibody NRG1 antibody MSTP131 antibody glial growth factor antibody sensory and motor neuron derived factor antib |
|---|---|
| Target | Neuregulin-1 |
| Molecular Weight(MW) | 70 kDa (note) (8) |
| Cellular Localization | Pro-neuregulin-1 , membrane-bound isoform: Cell membrane; Single-pass type I membrane protein , Neuregulin-1: Secreted , Isoform 8: Nucleus , Isoform 9: Secreted , Isoform 10: Membrane; Single-pass type I membrane protein |
Application
Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
U87-MG whole cell extract and conditioned medium (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Neuregulin-1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Neuregulin-1 antibody detects Neuregulin-1 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Neuregulin-1 protein stained by Neuregulin-1 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.
Application
Sample (50 µg of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Human brain , Mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
