Application
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Neurofibromin antibody diluted at 1:1000.| Product Name | Neurofibromin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Neurofibromin (neurofibromin 1)This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Neurofibromin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (98%), Rat (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | neurofibromin 1 WSS NFNS NF1 VRNF |
|---|---|
| Target | Neurofibromin |
| Molecular Weight(MW) | 319 kDa (note) (2) |
Application
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Neurofibromin antibody diluted at 1:1000.| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
