| Product Name | NF1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NF1ANuclear factor I is a ubiquitous 47-kD dimeric DNA-binding protein whose recognition sequence, TGG(C/A)N(5)GCCAA, is found in the genomes of a number of DNA viruses. The same sequence occurs frequently in the human genome. The NFI protein stimulates initiation of adenovirus DNA replication in vitro and is capable of stimulating the transcription of genes in cooperation with other factors, such as the estrogen receptor. Santoro et al. (1988) gave a detailed analysis of NFI cDNAs isolated from the human, which, with similar analyses in other species, indicated that it is a member of a family of related proteins. Four known variants were referred to as CTF, X, L, and Red. These are the products of different genes, some of which (e.g., the first, CTF, later symbolized NFIC) are subject to differential splicing. Seisenberger et al. (1993) isolated 2 phages containing the second exon and flanking intron regions of the human gene for NFI/X, which has been designated NFIX. Qian et al. (1995) isolated partial cDNA sequences from 4 nuclear factor I proteins: NFIA, NFIB, NFIC, and NFIX. |
| Immunogen | Synthetic peptide: NRFVSIGPRDGNFLN, corresponding to amino acids 478-492 of Human NF1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/500 - 1/1000. Detects bands of approximately 60 and 62 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | nuclear factor I/X (CCAAT-binding transcription factor) |
|---|---|
| Target | NF1A |
| Cellular Localization | Nuclear |
| Positive Control | Rat Liver nuclear extract |
|---|---|
| Application Notes | WB: Use at a dilution of 1/500 - 1/1000. Detects bands of approximately 60 and 62 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide and 0.01% Thiomerosal Constituents: PBS containing 0.2% Gelatin |
