Application
NFIB antibody [N1C2] detects NFIB protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. NFIB antibody [N1C2] dilution: 1:500.Application
NFIB antibody [N1C2] detects NFIB protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NFIB antibody [N1C2] dilution: 1:500.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Western Blot analysis of NFIB expression in transfected 293T cell line by NFIB polyclonal antibody. A: Non-transfected lysate. B: NFIB transfected lysate. 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | NFIB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFIB (nuclear factor I/B)Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NFIB. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, BovineRat (100%), Xenopus laevis (95%), Chicken (97%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HMGIC/NFIB antibody NFI-RED antibody NFIB2 antibody NFIB3 antibody NFIB antibody nuclear factor 1 B-type antibody CCAAT-box-binding transcription factor antibody TGGCA-binding protein antibody nuclear factor 1/B antibody nuclear factor I/B antibod |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
NFIB antibody [N1C2] detects NFIB protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. NFIB antibody [N1C2] dilution: 1:500.
Application
NFIB antibody [N1C2] detects NFIB protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NFIB antibody [N1C2] dilution: 1:500.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Western Blot analysis of NFIB expression in transfected 293T cell line by NFIB polyclonal antibody. A: Non-transfected lysate. B: NFIB transfected lysate. 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | nuclear factor I/B tansfected 293T cell , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
