Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using NFIC antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:2000| Product Name | NFIC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFIC (nuclear factor I/C (CCAAT-binding transcription factor))Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NFIC. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, BovineMouse (96%), Rat (96%), Xenopus laevis (83%), Chicken (89%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CTF antibody CTF5 antibody MGC20153 antibody NF-I antibody NFI antibody NFIC antibody NF1-C antibody CCAAT-box-binding transcription factor antibody TGGCA-binding protein antibody NF-I/C antibody nuclear factor 1 C-type antibody nuclear factor I |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (6) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using NFIC antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:2000| Positive Control | A549 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
