Application
WB analysis of extract from untreated and TNF alpha-treated MDA-MB-435 cells using NFkB p100 (phospho Ser870) antibody.Application
IHC-P analysis of human breast carcinoma using NFkB p100 (phospho Ser870) antibody.Application
ICC/IF analysis of methanol-fixed HeLa cells using NFkB p100 (phospho Ser870) antibody (red) and DAPI (blue).| Product Name | NFkB p100 (phospho Ser870) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFkB p100 (phospho Ser870)This gene encodes one of the subunits of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB transcription factor complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The NFkB complex can consist of different subunits that form both homo- or heterodimers which bind specific kappa-B elements in target genes. This gene encodes the p100 subunit that is processed into the active p52 subunit. This protein can function as both a transcriptional activator and repressor, depending on its dimer partner. Alternate splicing results in both coding and non-coding variants. [provided by RefSeq, May 2012] |
| Immunogen | A phospho specific peptide corresponding to residues surrounding S870 of human NFKB2 |
| Modification | p-Ser870 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:100. IF: 1:20 - 1:50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NF-kB2 p105 LYT-10 p52 nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) LYT10 NFKB2 H2TF1 |
|---|---|
| Target | NFkB p100 (phospho Ser870) |
| Molecular Weight(MW) | 120 kDa (note) |
Application
WB analysis of extract from untreated and TNF alpha-treated MDA-MB-435 cells using NFkB p100 (phospho Ser870) antibody.
Application
IHC-P analysis of human breast carcinoma using NFkB p100 (phospho Ser870) antibody.
Application
ICC/IF analysis of methanol-fixed HeLa cells using NFkB p100 (phospho Ser870) antibody (red) and DAPI (blue).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:100. IF: 1:20 - 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
