Application
NFkB p105 antibody immunoprecipitates NFkB p105 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of NFkB p105 antibody The precipitated DNA was detected by PCR with primer set targeting to PPM1D promoter.Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NFkB p105 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NFkB p105 antibody at 1:100 dilution.| Product Name | NFkB p105 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFkB p105 (nuclear factor of kappa light polypeptide gene enhancer in B-cells 1)This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. [provided by RefSeq] |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Rhesus Monkey, BovineRat (89%), Dog (94%), Pig (93%), Rhesus Monkey (99%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686C01211 antibody EBP-1 antibody KBF1 antibody MGC54151 antibody NF-kappa-B antibody NF-kappaB antibody NFKB-p105 antibody p105 antibody NFKB1 antibody nuclear factor kappa-B DNA binding subunit antibody NF-kappabeta antibody nu |
|---|---|
| Molecular Weight(MW) | 105 kDa (note) (3) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
NFkB p105 antibody immunoprecipitates NFkB p105 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of NFkB p105 antibody The precipitated DNA was detected by PCR with primer set targeting to PPM1D promoter.
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NFkB p105 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NFkB p105 antibody at 1:100 dilution.| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
