Application
WB analysis of HeLa lysate using NFkB p105/p50 (phospho Ser337) antibody.Application
IHC-P analysis of human breast carcinoma using NFkB p105/p50 (phospho Ser337) antibody.Application
ICC/IF analysis of methanol-fixed HeLa cells using NFkB p105/p50 (phospho Ser337) antibody.| Product Name | NFkB p105/p50 (phospho Ser337) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFkB p105/p50 (phospho Ser337)This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
| Immunogen | A phospho specific peptide corresponding to residues surrounding S337 of human NFKB1 |
| Modification | p-Ser337 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:100. IF: 1:100 - 1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NFKB1 , NF-kappa-B , NFKB-p50 , p50 , NFKB-p105 , NFkappaB , p105 , EBP-1 , nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 , NF-kappaB , NF-kB1 , KBF1 |
|---|---|
| Target | NFkB p105 |
| Molecular Weight(MW) | 50120 kDa (note) |
Application
WB analysis of HeLa lysate using NFkB p105/p50 (phospho Ser337) antibody.
Application
IHC-P analysis of human breast carcinoma using NFkB p105/p50 (phospho Ser337) antibody.
Application
ICC/IF analysis of methanol-fixed HeLa cells using NFkB p105/p50 (phospho Ser337) antibody.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:100. IF: 1:100 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
