Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)Application
Western blot analysis using NFKB1 mouse mAb against K562 (1), Jurkat (2), A431 (3), Hela (4), THP-1 (5) and MCF-7 (6) cell lysateApplication
Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and bladder cancer tissues (right) using NFKB1 mouse mAb with DAB stainingApplication
Flow cytometric analysis of MCF-7 cells using NFKB1 mouse mAb (green) and negative control (purple| Product Name | NFkB p105/p50 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [5D10] to NFkB p105/p50This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
| Immunogen | The immunogen corresponding to human NFkB p105/p50 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 5D10 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NFKAPPAB P50 NFKB-P105 EBP-1 NF-KAPPA-B NF-KB1 P105 NF-KAPPAB NFKB-P50 KBF1 |
|---|---|
| Target | NFkB p105/p50 |
| Molecular Weight(MW) | 50 kDa/105 kDa (note) |
Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)
Application
Western blot analysis using NFKB1 mouse mAb against K562 (1), Jurkat (2), A431 (3), Hela (4), THP-1 (5) and MCF-7 (6) cell lysate
Application
Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and bladder cancer tissues (right) using NFKB1 mouse mAb with DAB staining
Application
Flow cytometric analysis of MCF-7 cells using NFKB1 mouse mAb (green) and negative control (purple| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
