Application
Western blot analysis of NF-kB in 15 ugs of HeLa lysate using NF-kB p65 antibody at 1:1000 dilution.| Product Name | NFkB p65 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NFkB p65NFkB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NFkB transcription factor complex is composed of two DNA binding subunits, NFkB p65 and p50, both of which share extensive N terminal sequence homology with the v-rel oncogene product. N terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA binding specificities of p50 and p65. |
| Immunogen | Synthetic peptide: ADMDFSALL - amino acids 538-546 of Human NFkB p65. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseDogMonkeyPigRatGorilla |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | p65 antibody NFKB3 antibody NFkB p65 antibody |
|---|---|
| Target | NFkB p65 |
| Tissue Specificity | p65 subunit of NFkB |
| Cellular Localization | Cytoplasmic and Nuclear |
Application
Western blot analysis of NF-kB in 15 ugs of HeLa lysate using NF-kB p65 antibody at 1:1000 dilution.| Positive Control | HeLa or Raw whole cell lysate |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 0.1 mg/ml BSA and 0.02% sodium azide |
