Application
NFkB p65 antibody immunoprecipitates NFkB p65 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p65 protein by 2.5 μg of NFkB p65 antibody 10% SDS-PAGE The immunoprecipitated NFkB p65 protein was detected by NFkB p65 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
NFkB p65 antibody detects NFkB p65 protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg C8D30 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract 10% SDS-PAGE NFkB p65 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFkB p65 protein stained by NFkB p65 antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.Application
NFkB p65 antibody detects NFkB p65 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract 7.5% SDS-PAGE NFkB p65 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
NFkB p65 antibody detects NFkB p65 protein by western blot analysis. A. 30 µg K562 whole cell lysate/extract 7.5% SDS-PAGE NFkB p65 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
NFkB p65 antibody detects NFkB p65 protein by western blot analysis. Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using NFkB p65 antibody at 1:500 dilution.Application
Sample(30 µg whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NFkB p65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | NFkB p65 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFkB p65 (v-rel reticuloendotheliosis viral oncogene homolog A (avian))NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | EMSAICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Chimpanzee, BovinePig (98%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC131774 antibody, NFKB3 antibody, p65 antibody, RELA antibody, nuclear factor NF-kappa-B p65 subunit antibody, transcription factor p65 antibody, NF-kappa-B p65delta3 antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) (5) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
NFkB p65 antibody immunoprecipitates NFkB p65 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p65 protein by 2.5 μg of NFkB p65 antibody 10% SDS-PAGE The immunoprecipitated NFkB p65 protein was detected by NFkB p65 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
NFkB p65 antibody detects NFkB p65 protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg C8D30 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract 10% SDS-PAGE NFkB p65 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFkB p65 protein stained by NFkB p65 antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
Application
NFkB p65 antibody detects NFkB p65 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract 7.5% SDS-PAGE NFkB p65 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
NFkB p65 antibody detects NFkB p65 protein by western blot analysis. A. 30 µg K562 whole cell lysate/extract 7.5% SDS-PAGE NFkB p65 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
NFkB p65 antibody detects NFkB p65 protein by western blot analysis. Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using NFkB p65 antibody at 1:500 dilution.
Application
Sample(30 µg whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NFkB p65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
