Application
Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents the isotype control. red represents the NF-kB (p65) antibody.Application
IHC analysis of human tonsil using p65 antibody at 15 µg/ml.Application
Western blot analysis of p65 using p65 antibody at 2 µg/ml in 30 µg of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.| Product Name | NFkB p65 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [112A1021] to NFkB p65The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T-cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer. |
| Immunogen | Synthetic peptide: GLLSGDEDFSSIAD, corresponding to amino acids 526-539 of Human NFkB p65. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 112A1021 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-PWB |
| For FACS: Use at a concentration of 2-5 μg/ml. For IHC-P: Use at an assay dependent dilution. For WB: Use at a concentration of 2-5 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Bovine, Chimpanzee, Dog, Gorilla, Monkey, PigBovine(100%), Gorilla (100%), Monkey (100%), Pig (100%), Dog (100%), Chimpanzee (100%) |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | MGC131774 NFKB3 p65 |
|---|---|
| Target | NFkB p65 |
Application
Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents the isotype control. red represents the NF-kB (p65) antibody.
Application
IHC analysis of human tonsil using p65 antibody at 15 µg/ml.
Application
Western blot analysis of p65 using p65 antibody at 2 µg/ml in 30 µg of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.| Positive Control | Ramos, Daudi, HeLa, NIH 3T3 |
|---|---|
| Application Notes | For FACS: Use at a concentration of 2-5 μg/ml. For IHC-P: Use at an assay dependent dilution. For WB: Use at a concentration of 2-5 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 0.05% BSA and 0.05% sodium azide |
