Application
Whole extract of NIH-3T3 cells was separated on SDS-PAGE and probed with Monoclonal Anti-NF-κB Clone: NF-12 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. 1:250 antibody 2. 1:500 antibody 3. 1:1,000 antibody| Product Name | NFkB p65 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [NF-12] to NFkB p65NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| Immunogen | Recombinant, mouse NF-kB p65 fragment (C-terminal 151 amino acids). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | NF-12 |
| Host Species | Mouse |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P65 RELA NFKB3 |
|---|---|
| Target | NFkB p65 |
| Cellular Localization | Cytoplasmic and Nuclear |
Application
Whole extract of NIH-3T3 cells was separated on SDS-PAGE and probed with Monoclonal Anti-NF-κB Clone: NF-12 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. 1:250 antibody 2. 1:500 antibody 3. 1:1,000 antibody| Positive Control | 3T3 whole cell extract |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Contains 15 mM sodium azide |
