Application
Western blot analysis of extracts of MCF7 cells using NEFL antibody.Application
Immunohistochemistry of paraffin-embedded human breast cancer using NEFL antibody at dilution of 1:200 (400x lens).Application
Immunohistochemistry of paraffin-embedded rat kidney using NEFL antibody at dilution of 1:200 (400x lens).Application
Immunohistochemistry of paraffin-embedded rat brain using NEFL antibody at dilution of 1:200 (400x lens).Application
Immunofluorescence analysis of HeLa cell using NEFL antibody. Blue: DAPI for nuclear staining.| Product Name | NF-L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 68kDa Neurofilament LightNeurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008] |
| Immunogen | Recombinant protein of human NEFL |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NFL NF68 PPP1R110 NEFL NF-L neurofilament light polypeptide CMT2E CMT1F |
|---|---|
| Target | 68kDa Neurofilament Light |
| Molecular Weight(MW) | 62 kDa (note) |
Application
Western blot analysis of extracts of MCF7 cells using NEFL antibody.
Application
Immunohistochemistry of paraffin-embedded human breast cancer using NEFL antibody at dilution of 1:200 (400x lens).
Application
Immunohistochemistry of paraffin-embedded rat kidney using NEFL antibody at dilution of 1:200 (400x lens).
Application
Immunohistochemistry of paraffin-embedded rat brain using NEFL antibody at dilution of 1:200 (400x lens).
Application
Immunofluorescence analysis of HeLa cell using NEFL antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
