Application
A. Western blot analysis on SH-SY5Y lysate using anti-NF-L RabMAb (cat. # ) dilution 1:50000| Product Name | NF-L antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [EP675Y] to NF-LNeuofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton . NFs are composed of three major proteins; NF-H, NF-L and NF-M and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200 kDa), NF-M the medium (160 kDa) and NF-L the light protein (68kDa) . NF-L self polymerizes and provides a scaffold for NFs to form via cross-bridging with NF-H or NF-M . NF-L aggregation has been linked to the pathogenesis of neurodegenerative disease, such as degradation of motor neurons . |
| Immunogen | A synthetic peptide corresponding to residues near the C-term of human NF-L was used as immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP675Y |
| Host Species | Rabbit |
| Tested Applications | FACSIPWB |
| For WB: Use at a dilution of 1:1,000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | NF68 NEFL CMT2E NFL CMT1F NF-L FLJ53642 NF L |
|---|---|
| Target | NF-L |
| Molecular Weight(MW) | 68 (note) |
Application
A. Western blot analysis on SH-SY5Y lysate using anti-NF-L RabMAb (cat. # ) dilution 1:50000| Application Notes | For WB: Use at a dilution of 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
