Application
Indirect ELISA of adsorbed neurotrophic factors was performed. Neurotrophic factors mouse NGF 2.5S, mouse NGF 7S, hI2-NGF, proNGF, hBDNF, hNT-3 and hNT-4 were adsorbed to a microplate and analyzed using NGF/proNGF Neutralizing antibody, followed HRP-conjudated secondary antibody and chromogenic substrate.Application
Indirect ELISA of adsorbed proNGF and NGF was performed. Increasing amounts of proNGF (blue) and mouse NGF 2.5S (red) were adsorbed to a microplate and detected with NGF/proNGF Neutralizing antibody, followed by HRP-conjudated secondary antibody and chromogenic substrate.Application
Neutralization effect on NGF-mediated PC12 proliferation was analyzed in the absence of serum and in the presence of mouse NGF 2.5S (50 ng/ml) as indicated. Varying concentrations of NGF/proNGF Neutralizing antibody were added to the culture and cell viability was established after 24 h. Note that 600 ng/ml NGF/proNGF Neutralizing antibody was able to completely block the activity of 50 ng/ml mouse NGF.Application
Neutralizing assay of NGF-induced PC12 cells was performed (A) in the absence of NGF, (B) in the presence of mouse NGF 2.5S (50 ng/ml) and (C) in the presence of mouse NGF 2.5S (50 ng/ml) with NGF/proNGF Neutralizing antibody (1000 ng/ml). The neurite outgrowth over a period of 6 days was visualized by Methylene Blue staining.Application
Neutralizing assay of proNGF-induced PC12 cells was performed (A) in the absence of proNGF, (B) in the presence of proNGF (100 ng/ml) and (C) in the presence of proNGF (100 ng/ml) with NGF/proNGF Neutralizing antibody (500 ng/ml). The neurite outgrowth over a period of 6 days was visualized by Methylene Blue staining.| Product Name | NGF/proNGF Neutralizing antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [HAL06] to NGF/proNGF Neutralizing |
| Immunogen | Highly purified 2.5 S mouse NGF (Accession P01139). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | HAL06 |
| Host Species | Mouse |
| Tested Applications | ELISANeut |
| Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Ngf Ngfb nerve growth factor |
|---|---|
| Target | NGF/proNGF Neutralizing |
Application
Indirect ELISA of adsorbed neurotrophic factors was performed. Neurotrophic factors mouse NGF 2.5S, mouse NGF 7S, hI2-NGF, proNGF, hBDNF, hNT-3 and hNT-4 were adsorbed to a microplate and analyzed using NGF/proNGF Neutralizing antibody, followed HRP-conjudated secondary antibody and chromogenic substrate.
Application
Indirect ELISA of adsorbed proNGF and NGF was performed. Increasing amounts of proNGF (blue) and mouse NGF 2.5S (red) were adsorbed to a microplate and detected with NGF/proNGF Neutralizing antibody, followed by HRP-conjudated secondary antibody and chromogenic substrate.
Application
Neutralization effect on NGF-mediated PC12 proliferation was analyzed in the absence of serum and in the presence of mouse NGF 2.5S (50 ng/ml) as indicated. Varying concentrations of NGF/proNGF Neutralizing antibody were added to the culture and cell viability was established after 24 h. Note that 600 ng/ml NGF/proNGF Neutralizing antibody was able to completely block the activity of 50 ng/ml mouse NGF.
Application
Neutralizing assay of NGF-induced PC12 cells was performed (A) in the absence of NGF, (B) in the presence of mouse NGF 2.5S (50 ng/ml) and (C) in the presence of mouse NGF 2.5S (50 ng/ml) with NGF/proNGF Neutralizing antibody (1000 ng/ml). The neurite outgrowth over a period of 6 days was visualized by Methylene Blue staining.
Application
Neutralizing assay of proNGF-induced PC12 cells was performed (A) in the absence of proNGF, (B) in the presence of proNGF (100 ng/ml) and (C) in the presence of proNGF (100 ng/ml) with NGF/proNGF Neutralizing antibody (500 ng/ml). The neurite outgrowth over a period of 6 days was visualized by Methylene Blue staining.| Application Notes | Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) and pH 7.4. |
