Application
Western blot analysis of Nhe-1 in rat kidney tissue lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 ug/ml.Application
Immunohistochemical staining of human brain tissue using Nhe-1 antibody at 2.5 ug/ml.Application
Immunofluorescence of Nhe-1 in Human Brain cells with Nhe-1 antibody at 20 ug/mL.| Product Name | Nhe-1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Nhe-1The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Nhe1 Sodiumhydrogen exchanger 1 Nhe-1 Na+/H+ antiporter solute carrier family9 member1 Sodiumhydrogen exchanger1 Sodium-hydrogen exchanger 1 solute carrier family 9 member 1 SLC9A1 Nhe 1 Sodium hydrogen exchanger 1 |
|---|---|
| Target | Nhe-1 |
| Tissue Specificity | This antibody is specific for Nhe-1 |
Application
Western blot analysis of Nhe-1 in rat kidney tissue lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 ug/ml.
Application
Immunohistochemical staining of human brain tissue using Nhe-1 antibody at 2.5 ug/ml.
Application
Immunofluorescence of Nhe-1 in Human Brain cells with Nhe-1 antibody at 20 ug/mL.| Positive Control | Blocking Peptide: Cat.No. GTX85047-PEP - Nhe-1 Peptide Lysates: Cat. No. GTX27944 - Rat Kidney Tissue Lysate |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
