Application
Western blot analysis of NHE3 in rat kidney tissues using a 1:1000 dilution of| Product Name | NHE3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NHE3Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the prroximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine. NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro. |
| Immunogen | AA809 to 831 of rat sequence |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| We recommend a working dilution of 1:1000 (WB).: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Slc9a3 |
|---|---|
| Target | NHE3 |
| Molecular Weight(MW) | 84kDa (note) |
| Cellular Localization | In intestinal epithelial cells, localizes to the ileal brush border. |
Application
Western blot analysis of NHE3 in rat kidney tissues using a 1:1000 dilution of| Application Notes | We recommend a working dilution of 1:1000 (WB).: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20 C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | Rabbit immunoglobulin in 50% glycerol |
