Application
NHERF-2 antibody detects NHERF-2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10 % SDS-PAGE, and blotted with NHERF-2 antibody diluted by 1:1000Application
Sample (50 ug of whole cell lysate) A: mouse liver 15% SDS PAGE diluted at 1:1000| Product Name | NHERF2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NHERF2 (solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2)NHE3 (SLC9A3; MIM 182307) is a sodium/hydrogen exchanger in the brush border membrane of the proximal tubule, small intestine, and colon that plays a major role in transepithelial sodium absorption. SLC9A3R2, as well as SLC9A3R1 (MIM 604990) and protein kinase A phosphorylation, may play a role in NHE3 regulation.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NHERF-2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Rabbit, BovineRat (90%), Dog (95%), Pig (93%), Rabbit (83%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | E3KARP antibody MGC104639 antibody NHE3RF2 antibody NHERF-2 antibody NHERF2 antibody OCTS2 antibody SIP-1 antibody SIP1 antibody TKA-1 antibody SLC9A3R2 antibody sodium/hydrogen exchanger 3 kinase A regulatory protein antibody SRY-interacting pr |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) |
| Cellular Localization | Endomembrane system; Peripheral membrane protein , Nucleus |
Application
NHERF-2 antibody detects NHERF-2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10 % SDS-PAGE, and blotted with NHERF-2 antibody diluted by 1:1000
Application
Sample (50 ug of whole cell lysate) A: mouse liver 15% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
